Trypanosoma brucei

Trypanosoma brucei
Trypanosoma brucei brucei TREU667 (Bloodstream form, phase contrast picture. Black bar indicates 10 µm.)
Scientific classification
Kingdom: Excavata
Phylum: Euglenozoa
Class: Kinetoplastea
Order: Trypanosomatida
Genus: Trypanosoma
Species: T. brucei
Binomial name
Trypanosoma brucei
Plimmer & Bradford, 1899
Subspecies

T. b. brucei
T. b. gambiense
T. b. rhodesiense

Trypanosoma brucei is a parasitic protist species that causes African trypanosomiasis (or sleeping sickness) in humans and nagana in animals in Africa. There are 3 sub-species of T. brucei: T. b. brucei, T. b. gambiense and T. b. rhodesiense.

These obligate parasites have two hosts - an insect vector and mammalian host. Due to the large difference between these hosts the trypanosome undergoes complex changes during its life cycle to facilitate its survival in the insect gut and the mammalian bloodstream. It also features a unique and notable variable surface glycoprotein (VSG) coat in order to avoid the host's immune system. There is an urgent need for the development of new drug therapies as current treatments can prove fatal to the patient as well as the trypanosomes.

Contents

The infection: Trypanosomiasis

The insect vector for T. brucei is the tsetse fly. The parasite lives in the midgut of the fly (procyclic form), whereupon it migrates to the salivary glands for injection to the mammalian host on biting. The parasite lives within the bloodstream (bloodstream form) where it can reinfect the fly vector after biting. Later during a T. brucei infection the parasite may migrate to other areas of the host. A T. brucei infection may be transferred human to human via bodily fluid exchange, primarily blood transfer.

There are three different sub-species of T. brucei, which cause different variants of trypanosomiasis.

The cell structure

The structure of the cell is fairly typical of eukaryotes, see eukaryotic cell. All major organelles are seen, including the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus etc. Unusual features include the single large mitochondria with the mitochondrial DNA structure known as the kinetoplast, and its association with the basal body of the flagellum. The cytoskeleton is made up primarily of microtubules. The cell surface of the bloodstream form features a dense coat of variable surface glycoproteins (VSGs) which is replaced by an equally dense coat of procyclins when the parasite differentiates into the procylic in the tsetse fly midgut.

Trypanosomatids show specific cellular forms:

These names are derived from the Greek mastig- meaning whip, referring to the trypanosome's whip-like flagellum.

T. brucei is found as a trypomastigote in the slender, stumpy, procyclic and metacyclic forms. The procylic form differentiates to the proliferitive epimastigote form in the salivary glands of the insect. Unlike some other trypanosomatids, the promastigote and the amastigote form do not form part of the T.brucei life cycle.

The genome

The genome of T. brucei is made up of[3]:

The large chromosomes contain most genes, while the small chromosomes tend to carry genes involved in antigenic variation, including the VSG genes. The genome has been sequenced and is available online [1].

The mitochondrial genome is found condensed into the kinetoplast, an unusual feature unique to the kinetoplastea class. It and the basal body of the flagellum are strongly associated via a cytoskeletal structure.

VSG surface coat

Main section: The VSG coat

The surface of the trypanosome is covered by a dense coat of Variable Surface Glycoprotein (VSG), which allows persistence of an infecting trypanosome population in the host. See below.

The cytoskeleton

The cytoskeleton is predominantly made up of microtubules, forming a subpellicular corset. The microtubules lie parallel to each other along the long axis of the cell, with the number of microtubules at any point roughly proportional to the circumference of the cell at that point. As the cell grows (including for mitosis) additional microtubules grow between the existing tubules, leading to semiconservative inheritance of the cytoskeleton. The microtubules are orientated + at the posterior and - at the anterior.

Microfilament and intermediate filaments also play an important role in the cytoskeleton, but these are generally overlooked.

Flagellar structure

The trypanosome flagellum has two main structures. It is made up of a typical flagellar axoneme which lies parallel to the paraflagellar rod, a lattice structure of proteins unique to the kinetoplastida, euglenoids and dinoflagellates.

The microtubules of the flagellar axoneme lie in the normal 9+2 arrangement, orientated with the + at the anterior end and the - in the basal body. The a cytoskeletal structure extends from the basal body to the kinetoplast. The flagellum is bound to the cytoskeleton of the main cell body by four specialised microtubules, which run parallel and in the same direction to the flagellar tubulin.

The flagellar function is twofold - locomotion via oscilations along the attached flagellum and cell body, and attachment to the fly gut during the procyclic phase.

The VSG coat

The surface of the trypanosome is covered by a dense coat of ~1x107 molecules of Variable Surface Glycoprotein (VSG).[4] This coat enables an infecting T. brucei population to persistently evade the host's immune system, allowing chronic infection. The two properties of the VSG coat that allow immune evasion are:

Antigenic variation

Sequencing of the T. brucei genome has revealed a huge VSG gene archive, made up of thousands of different VSG genes. All but one of these are 'silent' VSGs, as each trypanosome expresses only one VSG gene at a time. VSG is highly immunogenic, and an immune response raised against a specific VSG will rapidly kill trypanosomes expressing this VSG. This can also be observed in vitro by a complement-mediated lysis assay. However, with each cell division there is a possibility that one or both of the progeny will switch expression to a silent VSG from the archive (see below). The frequency of such a switch has been measured to be approximately 1:100.[6] This new VSG will likely not be recognised by the specific immune responses raised against previously expressed VSGs. It takes several days for an immune response against a specific to develop, giving trypanosomes which have undergone VSG coat switching some time to reproduce (and undergo further VSG coat switching events) unhindered. Repetition of this process prevents extinction of the infecting trypanosome population, allowing chronic persistence of parasites in the host. The clinical effect of this cycle is successive 'waves' of parasitaemia (trypanosomes in the blood).[4]

VSG structure

VSG genes are hugely variable at the sequence level. However, for them to fulfil their shielding function, different VSGs have strongly conserved structural features. VSGs are made up of a highly variable N terminal domain of around 300 to 350 amino acids, and a more conserved C terminal domain of around 100 amino acids. The C terminal domain forms a structural bundle of four alpha helices, while the N terminal domain forms a 'halo' around the helices. The tertiary structure of this halo is well conserved between different VSGs (in spite of wide variation in amino acid sequence) allowing different VSGs to form the physical barrier required to shield the trypanosome's surface. VSG is anchored to the cell membrane via a glycophosphatidylinositol (GPI) anchor - a covalent linkage from the C terminus, to approximately four sugars, to a phosphatidylinositol phospholipid acid which lies in the cell membrane. VSGs form homodimers.

VSG archive structure

The VSG gene archive is the collection of silent VSGs in the T. brucei genome. Some of these are full-length, intact genes; others are pseudogenes) typically with omitted sections or premature stop codons. Expression of an antigenically novel VSG can occur by simply switching to a different full-length VSG gene. However, only 5% of the archive is made up of such complete silent VSGs. To utilise the rest of the silent VSG archive, ‘mosaic’ VSGs can be formed by replacing part of the expressed VSG with a structurally homologous region from the archive.[7] The combinatorial nature of mosaic formation in conjunction with the huge silent VSG archive gives the parasite a theoretically limitless VSG library, and is the major barrier to vaccine development.[8]

VSG expression

One major focus in trypanosome research is how the majority of VSG genes are kept silent, and how these genes are switched. The expressed VSG is always located in an Expression Site - found at the telomeres of the large and intermediate chromosomes. Each is a polycistronic unit, containing a number of Expression Site-Associated Genes (ESAGs) all expressed along with the active VSG. While there are at least 20 known expression sites, only a single one is ever active at one time. A number of mechanisms appear to be involved in this process, but the exact nature of the silencing is still unclear.[9]

The VSG can be switched either by changing the active expression (from the active to a previously silent site) or by changing the VSG gene in the active site. The genome contains many copies of possible VSG genes, both on minichromosomes and in repeated sections in the interior of the chromosomes. These are generally silent, typically with omitted sections or premature stop codons, but are important in the evolution of new VSG genes. It is estimated up to 10% of the T.brucei genome may be made up of VSG genes or pseudogenes. Any of these genes can be moved into the active site by recombination for expression. Again, the exact mechanisms that control this are still only partially known.

Cell division

The mitotic division of T.brucei is unusual compared to most eukaryotes. The nuclear membrane remains intact and the chromosomes do not condense during mitosis. The basal body, unlike the centrosome of most eukaryotic cells, does not play a role in the organisation of the spindle and instead is involved in division of the kinetoplast.

Stages of mitosis:

  1. The basal body duplicates and both remain associated with the kinetoplast.
  2. Kinetoplast DNA undergoes synthesis then the kinetoplast divides coupled with separation of the two basal bodies.
  3. Nuclear DNA undergoes synthesis while a new flagellum extends from the younger, more posterior, basal body.
  4. The nucleus undergoes mitosis.
  5. Cytokinesis progresses from the anterior to posterior.
  6. Division completes with abscission.

See also

List of parasites (human)

References

  1. ^ a b Barrett MP, Burchmore RJ, Stich A, et al. (November 2003). "The trypanosomiases". Lancet 362 (9394): 1469–80. doi:10.1016/S0140-6736(03)14694-6. PMID 14602444. 
  2. ^ Vanhamme L, Paturiaux-Hanocq F, Poelvoorde P, et al. (March 2003). "Apolipoprotein L-I is the trypanosome lytic factor of human serum". Nature 422 (6927): 83–7. doi:10.1038/nature01461. PMID 12621437. 
  3. ^ Ogbadoyi E, Ersfeld K, Robinson D, Sherwin T, Gull K (March 2000). "Architecture of the Trypanosoma brucei nucleus during interphase and mitosis". Chromosoma 108 (8): 501–13. doi:10.1007/s004120050402. PMID 10794572. http://link.springer.de/link/service/journals/00412/bibs/0108008/01080501.htm. 
  4. ^ a b Barry JD, McCulloch R (2001). "Antigenic variation in trypanosomes: enhanced phenotypic variation in a eukaryotic parasite". Adv Parasitol.. Advances in Parasitology 49: 1–70. doi:10.1016/S0065-308X(01)49037-3. ISBN 978-0-12-031749-3. PMID 11461029. 
  5. ^ Overath P, Chaudhri M, Steverding D, Ziegelbauer K (February 1994). "Invariant surface proteins in bloodstream forms of Trypanosoma brucei". Parasitol. Today (Regul. Ed.) 10 (2): 53–8. doi:10.1016/0169-4758(94)90393-X. PMID 15275499. http://linkinghub.elsevier.com/retrieve/pii/0169-4758(94)90393-X. 
  6. ^ Turner CM (August 1997). "The rate of antigenic variation in fly-transmitted and syringe-passaged infections of Trypanosoma brucei". FEMS Microbiol Lett. 153 (1): 227–31. doi:10.1111/j.1574-6968.1997.tb10486.x. PMID 9252591. http://linkinghub.elsevier.com/retrieve/pii/S0378-1097(97)00266-8. 
  7. ^ Marcello L, Barry JD (September 2007). "Analysis of the VSG gene silent archive in Trypanosoma brucei reveals that mosaic gene expression is prominent in antigenic variation and is favored by archive substructure". Genome Res. 17 (9): 1344–52. doi:10.1101/gr.6421207. PMC 1950903. PMID 17652423. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1950903. 
  8. ^ Barbour AG, Restrepo BI (2000). "Antigenic variation in vector-borne pathogens". Emerging Infect Dis. 6 (5): 449–57. doi:10.3201/eid0605.000502. PMC 2627965. PMID 10998374. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2627965. 
  9. ^ Pays E (November 2005). "Regulation of antigen gene expression in Trypanosoma brucei". Trends Parasitol. 21 (11): 517–20. doi:10.1016/j.pt.2005.08.016. PMID 16126458.